The inversion-recovery experiment allows to measure longitudinal or spin-lattice T1 relaxation times of any nucleus.REQUIREMENTS
Easy implementation on AVANCE spectrometers.VERSIONS
The basic pulse sequence of the inversion-recovery experiment consists of the following steps:EXPERIMENTAL DETAILS
The Inversion-recovery experiment can be acquired, processed and plotted in a fully automated way. Minor changes from a predefined parameter set are required. Important parameters to consider are:SPECTRA
- Relaxation delay must be set to 5*T1
- The experiment is repeated for a series of delays values.
- Rough estimation of the T1 value from the null-point value by using T1=tnull/ln(2). Quantitative analysis can be performed using the relaxation T1 /T2 software.
For further details on practical implementation of the inversion-recovery experiment on AVANCE spectrometers see:
The inversion-recovery experiment affords conventional 1D spectra. The signal intensities will shown a dependence with the interpulse t delay value:RELATED TOPICS
- Using a short delay, all signals will shown negative intensities (aligned to -z axis)
- The intensity of the signals will decrease (will be less negative) when the delay is increased
- The null-point (tnull) is achieved when the corresponding signal is missing (intensity zero)
- Using longer delays, the signal intensities become positive.
Related selective inversion-recovery experiments are efficient methods of measuring proton spin-lattice cross-relaxation rates in crowded regions ( 92MRC173 , 92JACS5412 , 93JB335 )